TABLE 2.
Strains used in this study
| Mycobacterium strain | Relevant features | Source |
|---|---|---|
| M. tuberculosis WT | ΔdevR/pUS Phsp60 devRwt, expresses WT DevR protein | This study |
| M. tuberculosis mutant | ΔdevR/pUS Phsp60 devRmut, expresses individual DevR mutant protein (D152A, E154A, etc.) | This study |
| Comp WT | ΔdevR electroporated with pUS Phsp60 devRwt and p1738 expressing WT DevR protein | This study |
| Comp XnA | ΔdevR electroporated with pUS Phsp60 devRD152A (or other mutants) and p1738; X and n represent amino acid residue and position, respectively, in WT DevR protein with alanine (A) substitution | This study |
| C-1738 | ΔdevR electroporated with pUS Phsp60 and p1738 (no source of DevR) | This study |
| M. smegmatis GCN4/GCN4 (positive) | Mycobacterium smegmatis mc2155 cotransformed with pUAB100 and pUAB200 | This study |
| M. smegmatis DevR/GCN4 (negative) | Mycobacterium smegmatis mc2155 cotransformed with pAV-FLdevR100 and pUAB200 | This study |
| M. smegmatis DevR/SigA | Mycobacterium smegmatis mc2155 cotransformed with pAV-FLdevR100 and pVS-SigA200 | This study |
| M. smegmatis E154A/SigA | Mycobacterium smegmatis mc2155 cotransformed with pAV-E154A and pVS-SigA200 | This study |
| M. smegmatis E178A/SigA | Mycobacterium smegmatis mc2155 cotransformed with pAV-E178A and pVS-SigA200 | This study |