FIG 7.
Effect of MTZ on the HsrA regulon. Shown is an immunoblot of MTZr strain HP1061 grown in the presence or absence of MTZ. Bacteria were grown to early logarithmic phase (OD600 of 0.3), at which time 10 μg/ml MTZ (+) was added to one flask, and the other served as a control (−). After 4 h, bacteria were collected, and cell extracts (4 to 32 μg/ml) were subjected to SDS-PAGE and probed with the indicated antibodies. GroEL (Hsp60) served as a control. The depicted immunoblot is representative of several replicates that also included different strains.