FIG 6.
Examination of the thermal stability of the Ph2119 endolysin. Samples of Ph2119 (25 μg/ml) endolysin and HEWL (25 μg/ml) were incubated in 10 mM KPO4, pH 8.0, at 95°C (0 to 17 h) or autoclaved (30 min; 121°C). Then, samples were placed on ice before measuring their activity against T. thermophilus HB8 cells at optimal temperature (60°C for Ph2119 endolysin and 37°C for HEWL). The activities of Ph2119 endolysin and HEWL are indicated as percentages relative to untreated samples. Each experiment was repeated in triplicate; the error bars indicate standard deviations.