FIG 3.

Relative copy number and expression of native and plasmid-associated rickA in transformed and wild-type R. bellii. Plasmid rickA was derived from R. monacensis, which allowed for differentiation of plasmid sequence from the native sequence. (A) Numbers of genetic copies of each type of rickA, normalized to the number of gltA copies. (B) Expression of native and plasmid rickA relative to gltA expression. Plasmid-derived rickA was only present above the threshold of detection in R. bellii_RmRickA. Relative gene copy number and expression of native rickA were comparable between all genotypes of R. bellii. Error bars represent standard errors.