TABLE 4.

Relative protein abundances of the enzymes metabolizing pyruvate during sulfate respiration and pyruvate fermentation by D. alaskensis G20 and CycA mutant I2^a

Reaction	Locus	Protein name	Annotationc	Lactate-sulfated			Pyruvatee
				I2 abundance	G20 abundance	Log-2 Rf (I2/G20)	I2 abundance	G20 abundance	Log2 R (I2/G20)
Ab	Dde_0182	GlcD	(S)-2-Hydroxy acid oxidase	7.30	6.52	0.16	0.42	NRg	Ih
	Dde_0312	COG-GlcD	FAD/FMN-containing dehydrogenase	11.13	9.23	0.27	2.46	6.74	−1.45
	Dde_0750	LdlD	Lactate dehydrogenase	14.58	11.82	0.30	20.09	11.74	0.77
	Dde_1087	COG-GlcD	FAD/FMN-containing dehydrogenase	NR	NR	NDi	NR	ND	ND
	Dde_3238	LldP	l-Lactate permease	4.23	4.12	0.04	1.04	1.32	−0.32
	Dde_3239	GlcD	Glycolate oxidase	445.77	441.43	0.01	407.13	508.48	−0.32
	Dde_3240	LdhB	Lactate dehydrogenase	34.22	31.51	0.12	19.34	11.13	0.79
B	Dde_2081	Pyc	Pyruvate carboxylase	21.66	21.05	0.04	6.70	6.32	0.08
C	Dde_1253	COG-SfcA	Malate:NADPH oxidoreductase, decarboxylating	0.40	40.38	−6.67	2.41	47.73	−4.31
	Dde_3637	Tme	Malic enzyme	8.93	11.63	−0.38	ND	53.20	Gj
D	Dde_0240	FumC	Fumarate hydratase	ND	ND	ND	ND	ND	ND
	Dde_1254	COG-FumA	Fumarate hydratase, β subunit	ND	30.30	G	ND	28.20	G
	Dde_3638	COG-FumA	Fumarate hydratase, β subunit	ND	NR	ND	NR	ND	ND
	Dde_3639	COG-TtdA	Fumarate hydratase, α region	ND	0.26	G	NR	ND	ND
E	Dde_1256	FrdB	Fumarate reductase, FeS protein	ND	26.64	G	NR	22.28	G
	Dde_1257	FrdA	Fumarate reductase, flavoprotein subunit	6.38	27.15	−2.09	NR	27.28	G
	Dde_1258	FrdC	Fumarate reductase, cytochrome b subunit	ND	12.55	G	ND	34.69	G
F	Dde_0473	FhcA	Putative cytoplasmic formate:hydrogen lyase, formate dehydrogenase subunit	ND	ND	ND	ND	ND	ND
	Dde_0474	FhcB	Putative cytoplasmic formate:hydrogen lyase, FeS subunit 1	ND	ND	ND	ND	ND	ND
	Dde_0475	FhcC	Putative cytoplasmic formate:hydrogen lyase, hydrogenase, small and large subunits	ND	ND	ND	ND	ND	ND
	Dde_0476	FhcD	Putative cytoplasmic formate:hydrogen lyase, FeS subunit 2	ND	ND	ND	ND	ND	ND
	Dde_0679	FdoG	Formate dehydrogenase, sulfur transferase	0.68	0.74	−0.11	11.37	4.34	1.39
	Dde_0680	FdoI	Formate dehydrogenase, cytochrome B556	ND	ND	ND	ND	ND	ND
	Dde_0681	FdoH	Formate dehydrogenase, FeS cluster	2.85	2.57	0.15	3.07	ND	I
	Dde_0716		Formate dehydrogenase, TAT pathway signal	35.29	0.48	6.21	114.55	ND	I
	Dde_0717	FdhA-1	Formate dehydrogenase, α subunit	46.97	2.04	4.53	119.83	0.33	8.57
	Dde_0718	FdhB-1	Formate dehydrogenase, β subunit	41.10	1.80	4.51	143.51	NR	I
	Dde_0812	FdhB-2	Formate dehydrogenase, β subunit	ND	ND	ND	ND	ND	ND
	Dde_0813	FdhA-2	Formate dehydrogenase, α subunit	22.38	NR	I	128.51	NR	I
	Dde_3513	FdhA-3	Formate dehydrogenase, α subunit	NR	0.36	G	9.62	ND	I
	Dde_3514	FdhB-3	Formate dehydrogenase, β subunit	ND	ND	ND	ND	ND	ND
G	Dde_3028	CooS	Carbon monoxide dehydrogenase	4.88	1.21	2.02	97.48	5.38	4.18
	Dde_3029	CooC	Carbon monoxide dehydrogenase	4.66	0.94	2.32	26.27	2.12	3.63
H	Dde_1792	PorA	Pyruvate-ferredoxin- oxidoreductase	NR	1.44	G	11.71	6.47	0.86
	Dde_1793	PorB	Pyruvate-ferredoxin- oxidoreductase	3.08	3.04	0.02	8.17	7.74	0.08
	Dde_3237	Por	Pyruvate-ferredoxin- oxidoreductase	232.69	224.03	0.05	118.14	116.82	0.02
I	Dde_3241	Pta	Phosphotransacetylase	69.06	67.97	0.02	21.88	26.32	−0.34
J	Dde_3242	AckA	Acetate kinase	70.66	88.75	−0.33	27.02	27.07	0.00

^aThe data presented here are supporting data for Fig. 3. The protein abundance values given in Fig. 3 were chosen for the most abundant isozyme for that reaction, and the locus, protein name, annotation, and data are highlighted in bold.

^bA through J designate the reactions illustrated in Fig. 3.

^cProtein annotation was obtained from http://www.microbesonline.org. FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide; TAT pathway, twin-arginine translocation pathway.

^dLactate-sulfate was MO basal salts with 60 mM sodium lactate and 30 mM sodium sulfate for sulfate respiration.

^ePyruvate was MO basal salts with 60 mM sodium pyruvate for growth by fermentation.

^fR, I2/G20 relative protein abundance values.

^gNR, data did not meet the criteria for reproducibility.

^hI, log₂ R was not meaningful since protein was detected only in the I2 culture.

ⁱND, no protein was detected in either G20 or I2 or the data did not meet the confidence criteria.

^jG, log₂ R was not meaningful since protein was detected only in the G20 culture.