FIG 3.

(A) Neighbor-joining tree showing the phylogenetic relationships between the 28 currently known ISE-SNP types of M. ulcerans, with haplotype ISE-SNP 28 from Papua New Guinea as an outgroup. Bootstrap values (if >70%) for the neighbor-joining (NJ), maximum likelihood (ML), and maximum parsimony (MP) analyses are indicated at the nodes as NJ/ML/MP. ISE-SNP types belonging to the pan-African clade and the Gabonese/Cameroonian clade are highlighted in gray and red, respectively. (B) Geographical distribution of the pan-African clade and the Gabonese/Cameroonian clade. The location of residence of the individual BU patients at the time of clinical visit was retrospectively correlated with the ISE-SNP typing results.