FIG 4.

Phylogenetic network showing patterns of descent among the 28 currently know ISE-SNP types of M. ulcerans in relation to their geographic origin. The network was derived by using the median joining algorithm after processing the data with the reduced median method as implemented within Network v.4.6.1.0. Each circle represents a unique ISE-SNP type, and the size of the circle is proportional to the number of individuals sharing that type. Numbers in boxes represent the number of mutational steps (if not given, then there was a single mutational step). Positions at which mutations occurred are given in Fig. 1. Color codes represent the country of origin, as shown in the key.