TABLE 3.
NEM modification of suicin and its mutants
| Suicin designation | Calculated molecular mass (Da) | Molecular mass by MS | PTMa | NEM addition (Da) | Molecular mass Δ (Da)b | No. of NEM |
|---|---|---|---|---|---|---|
| WT | 3,379.63 | 3,341.28 | 2H2O + S-S | 3,593.55 | 252.27 | 2 |
| T8A/T10A | 3,319.61 | 3,319.44 | –c | 3,819.87 | 500.43 | 4 |
| C13A | 3,347.66 | 3,309.47 | 2H2O | 3,561.55 | 250.08 | 2 |
| C21A | 3,347.66 | 3,311.52 | 2H2O | 3,436.58 | 125.06 | 1 |
| C30A | 3,347.66 | 3,311.53 | 2H2O | 3,436.60 | 125.07 | 1 |
| C33A | 3,347.66 | 3,309.52 | 2H2O | 3,561.64 | 250.12 | 2 |
| T10A/C33A | 3,317.65 | 3,299.64 | 1H2O | 3,549.69 | 250.05 | 2 |
a
PTM, posttranslational modification.
b
Data represent the mass difference after NEM addition.
c
–, no modification.