FIG 4.

SSU5 infects Salmonella mutants that lost their O-Ag and are resistant to O-Ag-specific phages. (A) Serially diluted SSU5 lysate was spotted onto the lawns of isolated P22H5- or SSU14-resistant mutants (indicated as P22-R[number] or SSU14-R[number], respectively). Phage titers used are the same as for Fig. 3B. (B) DOC-PAGE of LPS extracted from P22H5- or SSU14-resistant mutants. LPSs that were extracted by the hot-phenol extraction method were electrophoresed on a 15% polyacrylamide slab gel. Gels were stained using the Pro-Q Emerald 300 lipopolysaccharide gel stain kit prior to visualization under UV light. Lanes 1 and 11, Salmonella LPS standard (purchased from Sigma); lanes 2 and 12, WT LT2(c); lane 3, S. Typhimurium KCTC 1925 (see Table 1); lanes 4 and 13, ΔrfbP; lanes 5 to 10, P22-R1 to -R6 in panel A; lanes 14 to 19, SSU14-R1 to -R6 in panel A.