FIG 1.
Demonstration of suspensions of selected bacteria. The representative cultures used were F. hibernum (column 1), F. johnsoniae (column 2), B. thuringiensis (column 3), E. coli (column 4), and P. putida (column 5). These bacteria were larvicidal toxin producers or were used as hosts for toxin expression. Five-milliliter volumes of cells grown overnight were adjusted to an OD600 of 1.0, aliquoted into test tubes (12 by 75 mm), and incubated while stationary at room temperature. Pictures were taken every 2 days.