FIG 6.
Analysis of the digestibility of F. hibernum with a NanoLuc reporter by mosquito larvae. Third-instar larvae (4 days old) were starved for 2 h in sterile water before incubation with reporter strain SCH733 (ca. 2.8 × 109 cells/ml). The fed larvae were next rinsed and transferred into fresh sterile H2O for a digestion study. Larvae and the incubation solution were sampled at 0, 0.5, 1, 1.5, 2, 2.5, 3, and 4 h and subjected to the standard NanoLuc reporter assay as described in Materials and Methods. ◆, SCH733 cells in incubation solution; ●, SCH733 cells that remained in mosquito larvae.