TABLE 1.

Detection by real-time PCR of stx₁, stx₂, and eae genes and eae subtypes β1, ε, γ1, and θ in total DNA extracted from 150 cattle feces samples

Genetic marker(s) targeted by real-time PCR (either alone or in combination)	Positive samples (n = 150 tested)
	No.	%
Individual markers
stxa	133	88.7
stx1	80	53.3
stx2	109	72.7
eaeb	110	73.3
eae subtypec	88	58.7
eae-β1	49	32.7
eae-ε	10	6.7
eae-γ1	16	10.7
eae-θ	49	32.7
Combinations of markers
eae + eae subtype	84	56.0
stx + eae	100	66.7
stx1 + eae	17	11.3
stx2 + eae	37	24.7
stx1 + stx2 + eae	46	30.7
stx + eae subtype	79	52.7
stx + eae-β1	26	17.3
stx + eae-ε	3	2.0
stx + eae-γ1	2	1.3
stx + eae-θ	19	12.7
stx + eae-β1 + eae-ε	2	1.3
stx + eae-β1 + eae-γ1	2	1.3
stx + eae-β1 + eae-θ	12	8.0
stx + eae-ε + eae-θ	3	2.0
stx + eae-γ1 + eae-θ	7	4.7
stx + eae-β1 + eae-γ1 + eae-θ	3	2.0

^aSamples positive for stx were positive for stx₁ and/or stx₂.

^bDetection of the eae gene with universal primers/probe.

^cSamples positive for an eae subtype(s) were positive for at least one of the four targeted eae subtypes.