LC/ESI-MS analysis of DGGGP-Gro-derived archaeal phospholipid extracted from E. coli cells harboring pBAD-ALB4-MA1485/4. (A) Elution profiles of UV detection at 210 nm (top panel) and extracted ions (lower panels) in the LC/ESI-MS analysis of the lipid extracted from the E. coli strain Top10 harboring pBAD-ALB4-MA1485/4. The middle and bottom panels show the extracted ion profiles corresponding to [DGGGP-Gro+2Na]+ and [DPhGP-Gro+2Na]+, respectively. The ion peaks supposedly derived from archaeal lipids are marked by asterisks. (B) LC/ESI-MS analysis of lipid extracted from M. acetivorans was performed under the same conditions. The top, middle, and bottom panels show the elution profiles of UV detection at 210 nm, extracted ion corresponding to [DGGGP-Gro+2Na]+, and that corresponding to [DPhGP-Gro+2Na]+, respectively.