TABLE 2.

Primers used for plasmid construction and RT-PCR

Number	Primer name	Restriction site	Sequence (5′ → 3′)a
1	aphA3_F	SacI	AAC GAGCTC GGT ACC CGG GTG AC
2	aphA3_R	HincII	AAC GTCGAC TCT AGA GGA TCC CC
3	J166_IG_F1	HincII	AAT GTCGAC AAA TCT TAT TAT CGA CAC TCT ATT AGA A
4	J166_IG_R1	XhoI	AAT CTCGAG CCC TTT GTT GGC CGT CAA
5	J166_IG_F2	NotI	AAT GCGGCCGC TAG CAC CGC TCA AGA CAC AA
6	J166_IG_R2	SacI	AAT GAGCTC CCA AGT GAT AGT AAA GAT TAG GTA A
7	SS1_IG_F1	XhoI	AAT CTCGAG ATC ATA CCC CTA TTT CTA GGA
8	SS1_IG_R1	HincII	AAT GTCGAC AAA TCT TAT CAT CGA CAC TCT ATT AAA G
9	SS1_IG_R2	SacI	AAT GAGCTC TCA AGT GAT AGT AAA GAT TAG GTA G
10	cat_F	SacI	AAC GAGCTC GAT GCT TTA TAA CTA TGG ATT AAA CAC
11	cat_R	BamHI	AAC GGATCC TTA TCA GTG CGA CAA ACT GGG AT
12	J166_OMP_F1	BamHI	AAC GGATCC TTG CTC CAC GCT GAA GAC
13	J166_OMP_R1	XhoI	AAC CTCGAG GAC GCT CGT TTG ATT GAC CA
14	J166_OMP_F2	NotI	AAC GCGGCCGC AGC CAC AAA ACC TCT AAA GA
15	J166_OMP_R2	SacI	AAC GAGCTC GGG GTA TTT TGA AAT AAC TCT C
16	SS1_OMP_F1	XhoI	AAC CTCGAG TTT TGA GCC GGT GGA TAT ATT AG
17	SS1_OMP_R1	SacI	AAC GAGCTC GCA AGC TCT TTT ATT ATT TAT CTT A
18	SS1_OMP_F2	BamHI	AAC GGATCC CGT TAA AAC CTT TTG TGA AAC T
19	SS1_OMP_R2	NotI	AAA GCGGCCGC TGT GGA TCT AGC GGT TCT T
20	aphA3_F2	PstI	AAT CTGCAG GGT ACC CGG GTG AC
21	SS1_RecA_F1	NotI	AAT GCGGCCGC TCG TTA CTG CCC TTA ATG AGC TC
22	SS1_RecA_R1	PstI	AAT CTGCAG CAA TTT GTT TGA TCG CTA AAG AAA TCG C
23	SS1_RecA_F2	HincII	AAT GTCGAC AAT GAA GAG ATC ATG CCC TTA CCC
24	SS1_RecA_R1	XhoI	AAT CTCGAG AAAAGACAATCAGGGAGCTATGGC
25	comB4_F1		TGT TAG AAA AGC TTT TAA GCG C
26	comB4_R1		TTG CTC TGT GCT TAA ATG GGT G
27	comB9_F		AAG CCG ATG ATT TTT TAG AAG AAG C
28	comB9_R		AAA GCT CCC TTG AAT GGC GTT TAA
29	recA_F		GTG CGC CTT GGG GAT AAG CA
30	recA_R		CTT AGA GTG GTC TTC CCG CTT GAC
31	16S_F		GGA GTA CGG TCG CAA GAT TAA A
32	16S_F		CTA GCG GAT TCT CTC AAT GTC AA

^aUnderlining indicates a restriction site.