FIG 2.

Interaction of NsrR and ResD in vivo with selected transcription units during anaerobic fermentative growth. (A) NsrR ChAP-qPCR. B. subtilis strains OC0010 (wild type; white bars), ORB8278 (resD::cat; black bars), and ORB8277 (fur::kan; gray bars) carrying nsrR-his₁₂ were anaerobically grown until T1 in 2× YT supplemented with 0.5% glucose and 0.5% pyruvate. NsrR ChAP was performed and DNA associated with NsrR was purified as described in Materials and Methods. (B) ResD ChAP-qPCR. B. subtilis strains ORB8238 (wild type; white bars), ORB8264 (nsrR::cat; black bars), and ORB8266 (fur::kan; gray bars) carrying resD-his₁₂ were grown for ResD ChAP. ChAP-qPCR was carried out as described in Materials and Methods. Fold enrichment was calculated with triplicates of each biological sample normalized with rpsD as described in Materials and Methods, and the average for three biological samples is shown above each bar with the standard deviation.