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Effects of the resD, nsrR, and resD mutations on ykuN transcription. The wild-type and mutant strains carrying ykuN-lacZ were anaerobically grown in 2× YT supplemented with 1% glucose and 0.2% nitrate (A) (nitrate respiration) or 0.5% glucose and 0.5% pyruvate (B) (fermentation). Cells were harvested at hourly intervals, and β-galactosidase activities at T−1 (white bars) and T1 (gray bars) are shown. Error bars are standard deviations of data from triplicates.
