TABLE 1.

Mutants displaying P1 and P2 phenotypes^a

Mutant no.	P. aeruginosa IDb	Mutant IDc	Function	Pyocyanin production		Chloramphenicol resistance	Collagenase activity	Swarming motility
				Agar	Liquid
1	PA1003	ID13375	MvfR	−	−	+	−	−
2	PA5361	ID30430	PhoR	+	−	+	−	−
3	PA5394	PW10101	Cardiolipin synthase	−	+	−	+	+
4	PA0452	PW1826	Flotilin	−	+	−	+	+
5	PA2389	ID32408	PvdR	−	+	−	+	+
6	PA2391	ID9422	OpmQ	−	+	−	+	+
7	PA0685	ID39358	Probable type II secretion protein	−	+	−	+	+
8	PA0690	ID18158	Hypothetical protein	−	+	−	+	+
9	PA0691	ID4721	Hypothetical protein	−	+	−	+	+
10	PA0696	ID11082	Hypothetical protein	−	+	−	+	+
11	PA0051	ID39650	PhzH	−	+	−	+	+
12	PA0674	ID21628	VreA	−	+	−	+	+
13	PA0675	ID9556	ECF sigma factor - VreI	−	+	−	+	+
14	PA0676	ID16317	Sigma factor regulator - VreR	−	+	−	+	+
15	PA0678	ID42074	HxcU putative pseudopilin	−	+	−	+	+
16	PA0677	ID19639	HxcW putative pseudopilin	−	+	−	+	+
17	PA0678	ID54030	HxcU putative pseudopilin	−	+	−	+	+
18	PA0679	ID20782	Hypothetical protein	−	+	−	+	+
19	PA0680	ID35854	HxcV putative pseudopilin	−	+	−	+	+
20	PA0682	ID33753	HxcX atypical pseudopilin	−	+	−	+	+
21	PA5170	ID31482	ArcD	−	+	−	+	+
22	PA0688	ID12226	Probable binding protein component of ABC transporter	−	+	−	+	+
23	PA0681	ID5144	HxcT pseudopilin	−	+	−	+	+
24	PA4221	ID48243	FptA	−	+	−	+	+
25	PA2388	ID6650	FpvR	−	+	−	+	+
26	PA5360	ID40588	PhoB	−	+	−	+	+
27	PA2426	PW5085	Sigma factor, PvdS	−	+	−	+	+

^aChloramphenicol resistance is defined as growth on 500 ng/μl chloramphenicol (n = 2). Collagenase activity is defined as production of collagenase at 4 h of growth in TSB at levels at least 5 times higher than that of P1 (negatives had lower collagenase than P1) (n = 2). Mutant 1 (MvfR) does not produce pyocyanin because of a lack of quinolone production.

^bAs reported in the Pseudomonas Genome Database at http://www.pseudomonas.com/.

^cAs reported in the P. aeruginosa PAO1 transposon mutant library at http://www.gs.washington.edu/labs/manoil/libraryindex.htm.