FIG 3.

Localization of PilN, PilO, PilP, PilQ, and Tgl. Cells from exponentially growing cultures were transferred to a 1.5% agar pad on a microscope slide and imaged by fluorescence microscopy in the case of the fluorescent fusion proteins of PilO, PilP, PilQ, and Tgl. For native PilN, PilP, and Tgl, cells were fixed and imaged by immunofluorescence microscopy using anti-PilN, anti-PilP, and anti-Tgl antibodies, respectively. Upper and lower rows show fluorescence and phase-contrast (Ph) microscopy images, respectively. Scale bar, 5 μm.