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PilQ localizes polarly late during or immediately after cell division. M. xanthus cells from an exponentially growing culture were applied directly on a coverslip with a 0.1% CTT agarose pad and imaged by fluorescence and DIC microscopy at 20-min intervals. Arrows indicate newly formed polar clusters. Strain used, SA6060 (ΔaglZ/PpilA-tgl pilQ-sfGFP). The ΔaglZ mutation blocks gliding motility. Scale bar, 5 μm.
