TABLE 1.
M. xanthus strains used in this work
| M. xanthus strain | Relevant characteristicsa | Reference or source |
|---|---|---|
| DK1622 | Wild type | 51 |
| DK8615 | ΔpilQ | 81 |
| DK10409 | ΔpilT | 82 |
| DK10410 | ΔpilA | 83 |
| SA3002 | ΔpilM | 54 |
| DK10416 | ΔpilB | 82 |
| DK10417 | ΔpilC | 82 |
| DK10405 | Tcr::Δtgl | 33 |
| SA3046 | ΔpilM/PpilA-yfp-pilM (pSC8) | 54 |
| SA3045 | ΔpilT yfp pilT (pIB75) | 54 |
| SA3001 | ΔpilO | This work |
| SA3005 | ΔpilP | This work |
| SA3006 | ΔpilQ/PpilA-pilQ (pIB55) | This work |
| SA3007 | Δtgl/PpilA-tgl (pIB54) | This work |
| SA3044 | ΔpilN | This work |
| SA3053 | ΔpilM/PpilA-pilM (pSC2) | This work |
| SA4028 | ΔpilO/PpilA-pilO (pSC38) | This work |
| SA4029 | ΔpilP/PpilA-pilP (pSC39) | This work |
| SA4041 | ΔpilM/PpilA-yfp-pilMR388A (pSC56) | This work |
| SA4058 | ΔpilM/PpilA-yfp-pilMD204A (pSC97) | This work |
| SA4059 | ΔpilM/PpilA-yfp-pilMV204D (pSC98) | This work |
| SA4060 | ΔpilMNOPQ | This work |
| SA4061 | ΔpilN/PpilA-piN (pSC37) | This work |
| SA4067 | WT/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4070 | ΔpilP/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4076 | Δtgl/ PpilA-tgl-sfGFP (pSC104) | This work |
| SA4077 | ΔpilA/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4078 | ΔpilB/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4079 | ΔpilC/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4080 | ΔpilM/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4081 | ΔpilN/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4082 | ΔpilO/PpilA-pilP-sfGFP(pSC102) | This work |
| SA4083 | ΔpilT/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4086 | WT/PpilA-pilO-sfGFP (pSC106) | This work |
| SA4088 | ΔpilO/PpilA-pilO-sfGFP (pSC106) | This work |
| SA4094 | ΔpilQ/PpilA-pilP-sfGFP (pSC102) | This work |
| SA4095 | ΔpilM/PpilA-pilO-sfGFP (pSC106) | This work |
| SA4096 | ΔpilN/PpilA-pilO-sfGFP (pSC106) | This work |
| SA4097 | ΔpilP/PpilA-pilO-sfGFP (pSC106) | This work |
| SA4098 | ΔpilQ/PpilA-pilO-sfGFP (pSC106) | This work |
| SA6001 | ΔpilA/PpilA-pilO-sfGFP (pSC106) | This work |
| SA6002 | ΔpilB/PpilA-pilO-sfGFP (pSC106) | This work |
| SA6003 | ΔpilC/PpilA-pilO-sfGFP (pSC106) | This work |
| SA6005 | ΔpilT/PpilA-pilO-sfGFP (pSC106) | This work |
| SA6024 | ΔpilBTCMNOPQ | This work |
| SA6025 | ΔpilBTCMNOPQ/PpilA-yfp-pilT (pIB75) | This work |
| SA4073 | ΔpilTCMNOPQ | This work |
| SA6026 | WT/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6027 | ΔpilQ/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6028 | ΔpilA/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6029 | ΔpilB/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6030 | ΔpilN/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6031 | ΔpilP/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6033 | ΔpilC/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6034 | ΔpilM/PpilA-tgl pilQ-sfGF P (pSC120) | This work |
| SA6035 | ΔpilO/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6036 | ΔpilT/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6053 | Δtgl | This work |
| SA6056 | Δtgl/PpilA-pilP-sfGFP (pSC102) | This work |
| SA6057 | Δtgl/PpilA-pilO-sfGFP (pSC106) | This work |
| SA6058 | Δtgl/PpilA-yfp-pilT (pIB75) | This work |
| SA6060 | ΔaglZ/PpilA-tgl pilQ-sfGFP (pSC120) | This work |
| SA6061 | Δtgl/PpilA-pilQ-sfGFP (pSC110) | This work |
a
In constructs with PpilA, genes were transcribed from the pilA promoter. Plasmids containing the indicated expression constructs are in parentheses and were integrated at the Mx8 attB site.