FIG 5.
Comparison of the oxygen reductase activity and catalase activity between wild-type Cbb3-1, recombinant Cbb3-1, and Cbb3-2. Each bar represents the mean value ± SD, calculated from at least five independent measurements. The relative activity was calculated by assuming that the activity observed from wild-type Cbb3-1 was 100%. (A) Oxygen reductase activity was measured in the presence of 3 mM ascorbate, 1 mM TMPD, and 8.3 nM cbb3-CcO at pH 7.5. (B) Catalase activity was measured in the presence of 600 μM hydrogen peroxide and 500 nM cbb3-CcO.