FIG 6.

Nucleotide sequence (5′ to 3′) analysis of the upstream region of the ccoN-1 gene (A) and ccoNOQP-1 gene (B). An FNR (ANR) box (TTGAT-N⁴-gTCAA) is located upstream of the ccoN-1 transcription start site and is shown in blue. Sequences in the red boxes exhibit homology to the −10 and −35 promoter regions recognized by the σ⁷⁰-containing RNA polymerase. The RpoD (σ⁷⁰) recognition site in panel A is proposed based on the previously identified data from P. putida (43). The potential Shine-Dalgarno sequence is shown in green. Conserved nucleotides (compared to the consensus sequences) are in bold and capitalized. The translation initiation sites are indicated by arrows. The first six amino acids are underlined.