FIG 1.
Biofilm formation and flagellin synthesis in ymdB variants. (A) Colony surface architectures of individual colonies grown on MSgg medium are shown. The strains used were as follows: the wild-type (WT) strains (NCIB3610) and the ymdB (GP921), ymdB-cat (GP1618), and ymdBE39Q-cat (GP1619) mutants. The scale bar is 0.25 cm. (B) The expression levels of flagellin (Hag) of different strains are compared. Crude extracts were isolated from B. subtilis strains. Lane 1, 168 (wild type); lane 2, GP583 (ΔymdB::spc); lane 3, GP922 (ΔymdB::cat); lane 4, GP966 (ymdB-cat); lane 5, GP969 (ymdBE39Q-cat). The flagellin protein Hag is indicated with an arrow.