FIG 2.

Effect of YmdB on the sporulation genes sspB and sigG. (A) Northern blot analyses to test the effect of YmdB on the expression of sspB and sigG. The transcripts were detected with riboprobes specific for sspB and sigG. (B) The transcript stability of sspB and sigG mRNAs was determined for the wild type (168) and the ymdB mutant (GP583) by Northern blotting. Above each lane is the time (in minutes) after rifampin addition. The half-life of each mRNA transcript is also indicated in minutes. In the lane with the RNA from the ymdB strain, larger amounts of sspB and sigG mRNAs are detectable, but these mRNAs are not stabilized in comparison to the wild type.