FIG 6.

Examination of Crumbs3 knockout kidney. (A) Paraffin sections from E18.5 kidneys were stained for Crb3 (brown) and hematoxylin (blue). (B) Paraffin-embedded kidneys from wild-type E18.5 mice were stained for Crb3, Aqp1, and Aqp2 (brown) using serial sections. Red arrows indicate areas where weak Crb3-positive tubules were overlaid with Aqp1. Black arrows indicate the overlap of strongly Crb3-positive tubules with Aqp2. Nuclei were counterstained with hematoxylin (blue). (C) Paraffin sections from E18.5 kidneys were stained with H&E. (D) Paraffin-embedded kidneys from E18.5 mice were stained for H&E.