Pks5 positive and negative predictive values and cutoff values based on bovine tuberculosis prevalence. Positive and negative predictive values were plotted against percent prevalence values. Cutoff values were assigned to 4 scenarios based on prevalence. Scenario 1, high bTB prevalence (red); scenario 2, moderate bTB prevalence (blue); scenario 3, moderate-low bTB prevalence (yellow); scenario 4, low bTB prevalence (green); Pr, probability; D(+/−), Disease or no disease; T(+/−), test result positive or negative.