TABLE 1.
MST primers used for the PCR amplification and sequencing of L. interrogans isolates
| Spacer | ORF upstream (F) and downstream (R) of spacer sequencea | Position and nucleotide sequence (5′→3′) | PCR product size (bp)a |
|---|---|---|---|
| MST1 F | Hypothetical protein | 210612 ACG GGA GAA TCT GTC AAA GG | 207–263 |
| MST1 R | Hypothetical protein | 210837 AAA TTC ACT GAT TAC CTC CAC CA | |
| MST3 F | 4-Diphosphocytidil-2-C-methyl-d-erythriol kinase | 3792625 ACG ATC GTG CCA ATG TTT TT | 361–375 |
| MST3 R | Transcriptional regulator | 3793059 AAA ATT CAA ACC GGC AAA TG | |
| MST4 F | Hypothetical protein | 3656262 GGT TTA TTT GAA ATC ATG AAA ACT TCT | 323–328 |
| MST4 R | Hypothetical protein | 3656586 TAT CTT TTA TTC AAT CTT GTG AAT CC | |
| MST7 F | Inhibitor of MCP methylation | 2448482 AAA AGG CCT TCT TGG GTT TA | 392–393 |
| MST7 R | Hypothetical protein | 2448875 GAT CAA CGG TCC GTA AAA CG | |
| MST9 F | Bifunctional 3,4-dihydroxy-2-butanone 4-phosphate | 1155922 TTT GAA AAA CGA CTG AGG TCA A | 236–237 |
| MST9 R | Hypothetical protein | 1156167 TCG ATA TCG GCA CAA AAT CA |
a
ORF, open reading frame; F, forward; R, reverse. Based on the L. interrogans serovar Lai strain Lai 56601 genome sequence reference (GenBank accession no. AE010300.2).