TABLE 1.
Mutations and their locations identified in S. tigurinus SCV strains 2425 and 2426 relative to parental strain 1366
| Strain 1366 contig GenBank accession no. | Position | Mutation | Effect | Locus tag(s) | Gene product(s) |
|---|---|---|---|---|---|
| AORX01000001 | 20263 to 23836 | Inversiona | Unknown | H353_00090 and H353_00095 | Serine hydroxymethyltransferase and predicted ATPase |
| AORX01000001 | 27927 | G→A | Synonymous | H353_00130 | Hypothetical protein |
| AORX01000001 | 64202 | •→T | Frameshift | H353_00315 | Homoserine kinase ThrB |
| AORX01000001 | 138651 | A→C | 104 F→L | H353_00685 | Amino acid ABC transporter ATP-binding protein |
| AORX01000001 | 159403 | T→C | Synonymous | H353_00795 | Uracil phosphoribosyltransferase (Upp) |
| AORX01000001 | 210055 | C→T | 162 A→V | H353_01045 | Pyridine nucleotide-disulfide oxidoreductase |
| AORX01000001 | 494595 | C→A | 98 C→F | H353_02420 | Acetyltransferase, GNAT family protein |
| AORX01000001 | 615189 | T→C | Intergenic | ||
| AORX01000001 | 665652 | G→A | 102 R→STOP | H353_03270 | Zn2+-responsive transcriptional repressor AdcR |
| AORX01000001 | 754073 | A→C | 85 S→R | H353_03690 | β-Lactamase |
| AORX01000001 | 758016 | C→T | 364 A→V | H353_03705 | Cell division protein FtsH |
| AORX01000002 | 34504 | C→T | Intergenic | ||
| AORX01000002 | 92064 | C→T | 61 S→F | H353_04258 | 4′-Phosphopantetheinyl transferase AcpS |
| AORX01000002 | 235844 | A→T | 58 I→F | H353_04923 | Hypothetical protein |
| AORX01000002 | 454207 | A→C | 182 D→A | H353_06053 | F0F1- ATP synthase alpha subunit |
| AORX01000002 | 501697 | C→G | 530 S→STOP | H353_06288 | Phosphoenolpyruvate-protein phosphotransferase |
| AORX01000002 | 610271 | •→T | Frameshift | H353_06833 | ATPase component of ABC transporter |
| AORX01000002 | 670375 | T→C | Synonymous | H353_07143 | 3-Isopropylmalate dehydrogenase |
| AORX01000002 | 708850 | A→• | Intergenic | ||
| AORX01000003 | 16071 | •→T | Frameshift | H353_07674 | Glutamine synthetase |
| AORX01000003 | 55915 | C→T | Synonymous | H353_07859 | Hypothetical protein |
| AORX01000003 | 75333 | G→A | 243 A→V | H353_07954 | Peptide ABC transporter permease |
| AORX01000005 | 31194 | A→G | 297 R→G | H353_08704 | Maltodextrin ABC transporter permease |
a
Full 3.5-kbp inversion; both H353_00090 and H353_00095 are complete, and the next gene (H353_00100) is annotated as tyrosine recombinase. •, deleted position.