FIG 11.
Cell-mediated immunity of mice immunized with recombinant P. vivax CSP antigens. C57BL/6 mice were immunized as described in the legend to Fig. 10. Splenic cells of these mice were cultured in the presence or absence of the peptides or ConA, as indicated. The PvCS-VK210, PvCS-VK247, and PvCS-Vivax-like peptides represent the repeat regions of the allelic variants. After 48 h, IFN-γ-secreting cells (spot-forming cells [SFC]) were estimated by ELISPOT assay. The results are expressed as the frequency of SFC per 106 spleen cells obtained from pooled cells of five mice. The asterisk denotes a statistically significant difference (P < 0.01).