FIG 4.

In vitro screening of S100 alarmin response by PRR-deficient vaginal epithelial cells. Primary vaginal epithelial cell cultures derived from wild-type, MR^−/−, TLR4-deficient, and SIGNR1^−/− vaginal tissue explants were inoculated with C. albicans, and S100A8 (A) and S100A9 (B) mRNA expression was quantified by real-time PCR 24 h following inoculation. The expression of the target genes was normalized to that of the β-actin gene and expressed as the fold increase over expression in epithelial cells cultured alone in parallel. The figure presents cumulative data from two repeats. SEM, standard error of the mean.