FIG 6.

MyD88 is required for early inflammation in neonates after infection with Y. enterocolitica. (A) Neonatal wild-type C57BL/6 (wt) and MyD88^−/− mice were infected orogastrically with 5 × 10⁸ CFU Y. enterocolitica, and MLN from individual mice were evaluated for Ly6G⁺ CD11b⁺ and Ly6G⁻ CD11b⁺ cell recruitment by flow cytometry 24 h postinfection. Open symbols represent uninfected animals, and closed symbols represent infected animals. (B) Y. enterocolitica titers in the MLN and small intestine were determined 18 h postinfection. The level of detection is indicated by dotted lines. (C) RNA expression of Il6, Il17a, Ifng, and Cxcl1 relative to the lowest expressor 18 h postinfection. ns, not significant; *, P ≤ 0.05.