FIG 7.
The Y. enterocolitica ΔyopP strain elicits profound neutrophil recruitment to the neonatal MLN compared to adult MLN 48 h postinfection. (A) Neonatal and adult C57BL/6 mice were infected with 5 × 108 CFU wild-type Y. enterocolitica or the Y. enterocolitica ΔyopP strain, and Ly6G+ CD11b+ cell recruitment to the MLN of individual mice was evaluated by flow cytometry 24 and 48 h postinfection. (B) Representative flow profiles of MLN 48 h postinfection with the Y. enterocolitica ΔyopP strain are shown. (C) Wild-type Y. enterocolitica (●) and the Y. enterocolitica ΔyopP strain (○) titers in MLN were determined at 48 h postinfection. The dotted line represents the limit of detection of Y. enterocolitica CFU. ns, not significant; *, P ≤ 0.05.