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Loss of Gαi1 or Gαi3 mimics PTX to elicit HA sensitization. Mice deficient in Gαi/o subunits were analyzed for HA sensitivity in the absence of PTX exposure. Mice received 100 mg/kg HA i.v., and deaths were recorded within 30 min. Statistical significance was determined by one-way ANOVA using 8 to 23 mice per group. ***, P < 0.001.
