FIG 1.

TgPL1 localizes within the parasite in tachyzoites but on the cyst wall and PV space in bradyzoites. Tachyzoites were grown on coverslips containing confluent HFFs for 24 h before fixation. Bradyzoites were grown in confluent HFFs on coverslips under low CO₂ and high pH for 5 days. The parasites were stained for HA-tagged TgPL1 (green) and costained for dense-granule proteins GRA4 and GRA7, a cytoplasmic bradyzoite-specific heat shock protein (BAG1), or DBA as a marker of the bradyzoite cyst wall (all costain red). Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (blue in the merged images). DIC, differential interference contrast. Scale bar = 5 μm. The scale is identical for all bradyzoite images.