FIG 4.

Pneumococcal adhesion to airway cells (A549 cells) exposed to the supernatant of Prevotella intermedia (PiSup) in vitro. (A) Incubation with 5- to 10-fold-diluted PiSup increased Streptococcus pneumoniae CFU, indicating increased adhesion (*, P < 0.05 versus modified GAM broth control). The data are representative of three separate experiments. (B) Coinfection with a PAFR blocker (10 μM CV-3988) reduced PiSup-stimulated adhesion (*, P < 0.05 versus infection without PAFR blocker). The data are representative of three separate experiments. (C) PiSup increased PAFR transcript levels (*, P < 0.01 versus the broth control). The data are representative of two experiments with six replicates. All data represent means and SEM.