FIG 1.

Cytotoxicity and mouse mortality of V. vulnificus. (A) INT-407 cells were infected with the V. vulnificus strains at an MOI of 10. The cytotoxicity was determined by an LDH release assay and expressed using the total LDH released from the cells completely lysed by 1% Triton X-100 as 100%. Error bars represent the SEM. **, P < 0.005 relative to groups infected with the wild type at each incubation time. (B) Seven-week-old specific-pathogen-free female ICR mice were intragastrically infected with the wild type (WT) or the iscR mutant at doses of 10⁹ CFU. WT (pRK415), wild type; iscR (pRK415), iscR mutant; iscR (pJK1016), complemented strain.