FIG 5.

Adhesion of the V. vulnificus strains. (A) INT-407 cells were infected at an MOI of 10 with the V. vulnificus strains as indicated. After 30 min, adherent bacteria were enumerated, and results are presented as the numbers of bacteria per well of the tissue culture dishes. Error bars represent the SEM. **, P < 0.005 relative to the wild type. (B) INT-407 cells were infected with the V. vulnificus strains at an MOI of 10 for 30 min and morphologically observed using a light microscope after Giemsa staining (original magnification, ×1,200). The adhered V. vulnificus cells (filled arrowheads) and the cytoplasm of the INT-407 cells (open arrowheads) are indicated. WT (pRK415), wild type; iscR (pRK415), iscR mutant; iscR (pJK1016), complemented strain.