FIG 9.

Induction of iscR expression by H₂O₂. Total RNAs and proteins were isolated from wild-type V. vulnificus, grown anaerobically to an A₆₀₀ of 0.5, and then exposed to various levels of H₂O₂ for 10 min as indicated. (A) The iscR mRNA levels were determined by qRT-PCR analyses, and the iscR mRNA level in the bacteria not exposed to H₂O₂ (control) was set as 1. Error bars represent the SEM. **, P < 0.005 relative to the bacteria unexposed to H₂O₂. (B) Protein samples were resolved by SDS-PAGE; IscR was detected by Western blotting, and results are presented as described in the legend of Fig. 8.