TABLE 1.
Plasmids and bacterial strains used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Bacterial strains | ||
| V. vulnificus | ||
| M06-24/O | Clinical isolate; virulent | Laboratory collection |
| JK093 | M06-24/O with ΔiscR | This study |
| E. coli | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1; plasmid replication | Laboratory collection |
| S17-λpir | λ-pir lysogen; thi pro hsdR hsdM+ recA RP4-2 Tc::Mu-Km::Tn7;Tpr Smr; host for π-requiring plasmids, conjugal donor | 17 |
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) | Laboratory collection |
| Plasmids | ||
| pRL27 | Tn5-RL27; oriR6K; Kmr | 18 |
| pGEM-T Easy | PCR product cloning vector; Apr | Promega |
| pDM4 | R6K γ ori sacB; suicide vector; oriT of RP4; Cmr | 22 |
| pJK0909 | pDM4 with ΔiscR; Cmr | This study |
| pRK415 | IncP ori; broad-host-range vector, oriT of RP4; Tcr | 23 |
| pJK1016 | pRK415 with iscR; Tcr | This study |
| pET22b(+) | His6 tag fusion expression vector; Apr | Novagen |
| pJK0928 | pET22b(+) with iscR; Apr | This study |
a
Tpr, trimethoprim resistant; Smr, streptomycin resistant; Kmr, kanamycin resistant; Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Tcr, tetracycline resistant.