FIG 7.

Immunogenicity and protective efficacy of recombinant EatA passenger (rEatA_p) domain. (a to d) Kinetic ELISA data (expressed as V_max in milliunits/min) demonstrate antibody in samples from mice vaccinated with either LT alone (1 μg/dose), LT plus rEatA_p (1 μg LT plus 30 μg rEatA_p/dose), or PBS. The immunogen used is shown on the x axis of each graph. Serum (IgG, IgM, and IgA) responses (at 1:100 dilution of sera) to LT (a) and rEatA_p (b) and fecal (IgA) responses (undiluted samples) to rEatA_p (c) and LT (d) are shown. αLT, anti-LT antibody. (e) LT/rEatA_p-immunized mice were protected against colonization by ETEC following intestinal challenge with jf876 (data obtained 24 h after challenge; P = 0.0063 by Mann-Whitney two-tailed nonparametric analysis).