TABLE 1.
Bacterial strains and plasmids used in these studies
| Strain or plasmid | Descriptiona | Reference(s) or source |
|---|---|---|
| Strains | ||
| H10407 | Wild-type ETEC strain O78:H11; CFA/I; LT+/ST+; EtpA+; EatA+ | 30, 52 |
| TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) | Invitrogen |
| jf904 | H10407 eatA::Cmr mutant | 24 |
| jf876 | H10407 ΔlacZYA::Kmr | 39 |
| Plasmids | ||
| pBAD/Myc-HisA | Arabinose-inducible expression plasmid | Invitrogen |
| pSB001 | 7,064-bp BamHIΔlac subclone from pBAD-TOPO/lacZ/V5-His | 25 |
| pSP014 | 4,185-bp eatA amplicon cloned into pBAD-TOPO | 24 |
| pSP019 | pSP014 altered by SDM for His-134→Arg-134 substitution of EatA | 24 |
a
Strr, streptomycin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; SDM, site-directed mutagenesis.