FIG 5.
Syk, Dectin-1, and Card9 are involved in the secretion of IL-1β induced by M. canis infection in THP-1-derived macrophages. Syk-, Dectin-1-, or Card9-silenced and Scramble THP-1-derived macrophages (1 × 105) were infected with M. canis (MOI, 1) for 6 h, the culture supernatants were collected for IL-1β ELISA (A), and the macrophages were harvested for pro-IL-1β assay via qPCR (B). (C) The knockdown efficiencies of Syk, Dectin-1, and Card9 were well controlled. *, P < 0.05; **, P < 0.01. Data in panels A, B, and C are means ± standard deviations from one out of three independent experiments.