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. 2014 Feb;82(2):882–892. doi: 10.1128/IAI.01097-13

FIG 6.

FIG 6

Heat-sensitive components from M. canis contribute to pro-IL-1β induction and caspase-1 activation. (A) THP-1-derived macrophages (1 × 105) were treated with M. canis, the macrophages and fungus were harvested at various time points, total RNA was extracted, and M. canis 18S rRNA was detected using qPCR. THP-1 cells (1 × 105) were challenged with M. canis or 4% PFA-treated M. canis cells (25°C, 30 min) for 6 h, and the culture supernatants were harvested for IL-1β (B) and IL-8 (C) detection by ELISA. THP-1 cells (1 × 105) were challenged with M. canis or heat-killed M. canis (90°C, 30 min) for 6 h, and the culture supernatants were harvested for IL-1β (D) and IL-8 (E) detection by ELISA. (F) THP-1 cells (1 × 106) were stimulated with M. canis or heat-killed M. canis, and the cell lysates and supernatants were collected for pro-IL-1β and caspase-1 detection, respectively. *, P < 0.05; **, P < 0.01. Data in panel A are means ± standard deviations from one out of two independent experiments. Data in panels B, C, D, and E are mean ± standard deviations from one out of three independent experiments. Data in panel F are from one out of two independent experiments.