TABLE 1.
Bacterial strains and plasmids used in these studies
| Strain or plasmida | Descriptionb | Reference(s) or source |
|---|---|---|
| Strains | ||
| H10407 | Wild-type ETEC strain O78:H11; CFA/1; LT+/ST+; EtpA+ | 56, 57 |
| E24377A | WT ETEC strain O139:H28 | 58 |
| B7A | WT ETEC strain O148:H28 | 59 |
| Nissle 1917 | O6:H1 probiotic strain originally isolated from an asymptomatic German soldier | 32 |
| HS | Isolated from a healthy adult scientist at WRAIR; O9:H4; motile | 31 |
| jf1123 | H10407 derivative with isogenic deletion of gspE | 11 |
| jf1124 | H10407 derivative with isogenic deletion of gspM | 11 |
| jf1121 | H10407 derivative with isogenic deletion of gspG | 11 |
| TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) | Invitrogen |
| yghJ mutant | Isogenic insertion in yghJ; Kmr Cmr | 14 |
| Plasmids | ||
| pBAD/Myc-HisA | Arabinose-inducible expression plasmid | Invitrogen |
| pQL001 | yghJ cloned into XhoI and HindIII sites of pBAD/myc-HisA in frame with myc-His tags | This study |
| pQL117* | Site-directed mutation of yghJ in codon corresponding to E1309(D) | This study |
| pQL114* | Site-directed mutation of yghJ in codon corresponding to E1309(A) | This study |
| pQL150* | Site-directed mutation of yghJ in codon corresponding to H1308(A) | This study |
| pQL152* | Site-directed mutation of yghJ in codon corresponding to H1312(A) | This study |
| pQL124* | 66-nucleotide in-frame deletion in yghJ | This study |
a
Mutagenized yghJ plasmids (indicated by asterisks) used plasmid pQL001 as a template for mutagenesis.
b
WRAIR, Walter Reed Army Institute of Research; Strr, streptomycin resistant; Kmr, kanamycin resistant; Cmr, chloramphenicol resistant.