FIG 5.

Both ΔT-vaccinated WT and IL-10^−/− mice revealed elevated percentages and numbers of T_EM cells as well as IFN-γ-, IL-5-, and IL-17A-expressing CD4⁺ T cells compared to nonvaccinated mice. (A) Percentages of T_EM (CD4⁺ CD44⁺ CD62⁻) and T_CM (CD4⁺ CD44⁺ CD62L⁺) cells within the gated CD4⁺ T-cell population in the lungs of nonvaccinated (PBS-Ctl.) versus vaccinated (ΔT) mice are shown at 8 or 12 DPC. Numbers above the gates indicate the mean percentages of the gated T_EM and T_CM subsets. (B) The cell numbers of gated, specific cytokine-producing CD4⁺ T cells per lung were determined by intracellular cytokine staining. Asterisks indicate significantly higher cell numbers of responsive T-cell subpopulations in lungs of vaccinated compared to nonvaccinated mice, while the daggers indicate higher numbers of selected cytokine-producing cells in IL-10^−/− mice compared to WT counterparts. Error bars indicate standard errors. The results reported are representative of two independent experiments.