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MERS-CoV rRT-PCR assay limits of detection with RNA transcripts
| Predicted no. of copies/reaction | No. of positive tests/no. of transcript replicates (%) |
||
|---|---|---|---|
| upE | N2 | N3 | |
| 20 | 24/24 (100) | 24/24 (100) | 24/24 (100) |
| 10 | 24/24 (100)a | 24/24 (100) | 24/24 (100) |
| 5 | 18/24 (75) | 24/24 (100)a | 24/24 (100)a |
| 2.5 | 14/24 (58) | 21/24 (87.5) | 22/24 (91.7) |
| 1.25 | 8/24 (33) | 14/24 (58.3) | 16/24 (66.7) |
Highest dilution at which 100% of replicates were positive.
