TABLE 4.

MERS-CoV rRT-PCR assay limits of detection with culture-extracted MERS-CoV RNA

Virus quantity (TCID50/ml)	upEa				N2				N3
	CT			No. positive/no. tested	CT			No. positive/no. tested	CT			No. positive/no. tested
	Test 1	Test 2	Test 3		Test 1	Test 2	Test 3		Test 1	Test 2	Test 3
1.3 × 10−0	27.35	27.46	27.39	3/3	27.88	27.98	28.07	3/3	25.98	25.95	25.81	3/3
1.3 × 10−1	31.06	31.13	31.29	3/3	31.68	31.64	31.77	3/3	29.67	29.38	29.40	3/3
1.3 × 10−2	33.81	34.55	34.67	3/3	35.87	34.62	35.70	3/3	32.81	33.06	33.23	3/3
1.3 × 10−3	37.03	39.01	38.94	3/3	38.74	40.01	38.11	3/3	36.60	37.28	35.82	3/3
1.3 × 10−4	39.07	Neg	Neg	1/3	Neg	Neg	Neg	0/3	Neg	Neg	Neg	0/3
1.3 × 10−5	Neg	Neg	Neg	0/3	Neg	Neg	Neg	0/3	Neg	Neg	Neg	0/3
1.3 × 10−6	Neg	Neg	Neg	0/3	Neg	Neg	Neg	0/3	Neg	Neg	Neg	0/3

^aC_T, threshold cycle; Neg, negative.