TABLE 5.
MERS-CoV rRT-PCR assay performance with virus-spiked specimen pools
| Virus amount (TCID50/ml) | Specimen | upEa |
N2 |
N3 |
RP |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
CT |
No. positive/no. tested |
CT |
No. positive/no. tested |
CT |
No. positive/no. tested |
CT |
No. positive/no. tested | ||||||||||
| Test 1 | Test 2 | Test 3 | Test 1 | Test 2 | Test 3 | Test 1 | Test 2 | Test 3 | Test 1 | Test 2 | Test 3 | ||||||
| 1.3 × 100 | NP/OP | 26.08 | 26.35 | 26.32 | 3/3 | 25.88 | 25.92 | 25.86 | 3/3 | 23.38 | 23.50 | 23.51 | 3/3 | 27.62 | 27.66 | 27.72 | 3/3 |
| Sputum | 26.62 | 26.72 | 26.75 | 3/3 | 26.07 | 26.13 | 26.15 | 3/3 | 23.69 | 23.74 | 23.76 | 3/3 | 23.43 | 23.56 | 23.61 | 3/3 | |
| Serum | 26.81 | 26.71 | 27.07 | 3/3 | 26.55 | 26.46 | 26.48 | 3/3 | 25.71 | 25.66 | 25.65 | 3/3 | 31.53 | 31.02 | 31.04 | 3/3 | |
| Stool | 27.24 | 27.67 | 28.05 | 3/3 | 28.26 | 28.34 | 28.45 | 3/3 | 25.13 | 25.40 | 25.56 | 3/3 | 37.36 | 38.15 | 36.74 | 3/3 | |
| 1.3 × 10−1 | NP/OP | 27.90 | 27.86 | 27.85 | 3/3 | 27.35 | 27.24 | 27.49 | 3/3 | 25.00 | 25.16 | 24.93 | 3/3 | 27.58 | 27.62 | 27.69 | 3/3 |
| Sputum | 29.13 | 29.05 | 29.11 | 3/3 | 28.19 | 28.24 | 28.23 | 3/3 | 25.86 | 25.83 | 25.90 | 3/3 | 23.02 | 23.22 | 23.22 | 3/3 | |
| Serum | 30.18 | 30.76 | 30.75 | 3/3 | 30.11 | 30.26 | 30.23 | 3/3 | 29.30 | 29.26 | 29.37 | 3/3 | 31.19 | 31.04 | 31.42 | 3/3 | |
| Stool | 30.86 | 31.99 | 31.62 | 3/3 | 32.84 | 32.12 | 32.12 | 3/3 | 29.47 | 29.93 | 29.90 | 3/3 | 38.00 | 38.12 | 37.47 | 3/3 | |
| 1.3 × 10−2 | NP/OP | 34.04 | 34.51 | 34.25 | 3/3 | 34.31 | 33.98 | 34.14 | 3/3 | 31.95 | 31.92 | 31.90 | 3/3 | 27.84 | 27.88 | 27.75 | 3/3 |
| Sputum | 35.29 | 35.59 | 34.88 | 3/3 | 35.35 | 34.67 | 35.52 | 3/3 | 32.57 | 32.69 | 32.27 | 3/3 | 23.28 | 23.28 | 23.28 | 3/3 | |
| Serum | 33.62 | 33.67 | 33.54 | 3/3 | 33.03 | 33.00 | 33.33 | 3/3 | 32.70 | 32.43 | 32.18 | 3/3 | 31.29 | 30.98 | 31.19 | 3/3 | |
| Stool | 34.93 | 35.74 | 35.08 | 3/3 | 36.95 | 37.83 | 36.72 | 3/3 | 32.74 | 33.87 | 33.68 | 3/3 | 36.69 | 38.23 | 38.36 | 3/3 | |
| 1.3 × 10−3 | NP/OP | 38.70 | 37.87 | 39.36 | 3/3 | 37.50 | 37.11 | 38.10 | 3/3 | 36.57 | 35.43 | 35.41 | 3/3 | 27.87 | 28.01 | 28.03 | 3/3 |
| Sputum | 38.52 | 38.30 | Neg | 2/3 | 37.58 | 37.94 | 37.50 | 3/3 | 35.49 | 35.44 | 34.90 | 3/3 | 23.21 | 23.25 | 23.27 | 3/3 | |
| Serum | 40.76 | 37.86 | 37.35 | 3/3 | 36.63 | 37.04 | 37.99 | 3/3 | 36.78 | 36.69 | 36.60 | 3/3 | 31.32 | 31.09 | 31.36 | 3/3 | |
| Stool | 39.49 | Neg | 38.45 | 2/3 | 36.95 | 37.83 | 36.72 | 3/3 | 36.41 | 37.52 | 36.57 | 3/3 | 39.47 | 37.20 | 36.88 | 3/3 | |
| 1.3 × 10−4 | NP/OP | Neg | Neg | Neg | 0/3 | Neg | Neg | Neg | 0/3 | Neg | Neg | Neg | 0/3 | 28.01 | 28.00 | 27.96 | 3/3 |
| Sputum | Neg | Neg | Neg | 0/3 | Neg | Neg | Neg | 0/3 | Neg | Neg | Neg | 0/3 | 24.74 | 24.72 | 24.69 | 3/3 | |
| Serum | Neg | Neg | Neg | 0/3 | Neg | Neg | 39.72 | 1/3 | Neg | 40.44 | 39.58 | 2/3 | 31.40 | 31.28 | 31.65 | 3/3 | |
| Stool | Neg | Neg | Neg | 0/3 | Neg | Neg | Neg | 0/3 | Neg | Neg | Neg | 0/3 | 37.60 | 37.24 | 36.99 | 3/3 | |
a
Neg, negative.