TABLE 8.

MERS-CoV rRT-PCR assay performance with individual virus-spiked serum and stool specimens

Virus quantity (TCID50/ml)	Serum samples				Stool samples
	Sample no.	CT			Sample no.	CT
		upE	N2	N3		upE	N2	N3
1.3 × 10−1	SE1	32.19	32.66	31.74	ST1	34.63	33.95	32.68
	SE2	37.08	37.57	36.98	ST2	31.35	31.17	29.37
	SE3	34.10	35.02	33.88	ST3	34.43	33.51	32.48
	SE4	34.48	34.36	33.63	ST4	34.40	33.74	32.94
	SE5	36.30	36.28	35.86	ST5	33.98	33.70	32.29
	SE6	32.40	32.42	31.82	ST6	34.59	34.21	32.89
	SE7	33.28	33.28	32.60	ST7	34.07	33.43	32.16
	SE8	36.33	36.33	36.33	ST8	33.96	33.07	31.84
	SE9	33.92	34.31	33.02	ST9	32.32	32.27	30.78
	SE10	32.43	32.63	31.69	ST10	34.57	33.91	32.86
		34.25 (32.19–37.08)a	34.49 (32.42–37.57)a	33.76 (31.69–36.98)a		33.83 (31.35–34.63)a	33.30 (31.17–34.21)a	32.03 (29.37–32.94)a
1.3 × 10−2	SE11	35.56	35.93	35.78	ST11	37.08	37.10	35.89
	SE12	41.49	43.41	39.76	ST12	37.16	36.52	35.27
	SE13	36.38	37.96	38.96	ST13	37.36	36.16	35.54
	SE14	39.59	39.76	38.62	ST14	35.97	35.72	34.60
	SE15	36.47	36.12	35.75	ST15	37.76	37.10	36.50
	SE16	38.29	39.23	39.22	ST16	37.42	37.85	35.09
	SE17	38.52	38.08	38.11	ST17	36.96	37.00	36.11
	SE18	35.25	35.46	34.68	ST18	39.81	37.23	35.69
	SE19	38.18	40.11	39.23	ST19	37.53	37.39	36.00
	SE20	37.81	38.46	38.55	ST20	37.94	37.24	35.72
		37.75 (35.25–41.49)a	38.45 (35.46–43.41)a	37.87 (34.68–39.76)a		37.50 (35.97–39.81)a	36.93 (35.72–37.85)a	35.64 (34.60–36.50)a
No virus	SE21-70	Negb	Neg	Neg	ST21-70	Neg	Neg	Neg

^aMean (range).

^bNeg, negative.