TABLE 1.

C_T values of the A(H1N1)pdm09-positive clinical specimens given by two real-time RT-PCR assays

Specimen no.	CT values from the indicated assay		ΔCTc	HA clade A(H1N1)pdm09	Collection date (in 2013)
	Aw1a	Atb
95,316	34.92	31.00	3.92	12.2	28 Decemberd
95,380	UDe	32.62		NDf	7 March
95,402	32.85	25.74	7.11	12.1	14 March
95,409	34.53	30.12	4.41	ND	15 March
95,411	UD	34.95		ND	15 March
95,460	34.55	27.17	7.38	12.1	3 April
95,507	34.53	25.36	9.17	12.1	25 April
80,070	33.98	30.08	3.9	12.2	24 January
80,075	28.56	21.29	7.27	12.1	25 January
80,079	33.46	27.27	6.19	12.2	26 January
80,109	32.20	28.98	3.22	12.2	4 February
80,125	35.73	29.64	6.09	11.1	8 February
80,128	32.70	28.21	4.49	11.1	8 February
80,148	35.00	29.47	5.53	12.2	15 February
80,205	32.30	26.18	6.12	11.1	27 February
80,208	29.04	22.48	6.56	12.1	28 February
80,210	32.64	23.8	8.84	12	28 February
80,219	27.14	21.35	5.79	11.1	1 March
80,224	32.82	26.56	6.26	12.2	4 March
80,243	33.45	30.34	3.11	12.2	7 March
80,261	30.77	23.46	7.31	11.1	11 March
80,272	22.94	17.11	5.83	12.2	13 March
80,310	37.52	30.63	6.89	12.1	20 March
80,426	31.08	23.88	7.20	12.1	6 April
80,462	34.57	27.03	7.54	12.1	10 April
80,470	32.41	25.95	6.46	12.2	11 April
80,515	34.98	26.97	8.01	12.1	18 April
80,981	38.24	29.97	8.27	11.1	21 June
80,993	33.82	28.23	5.59	11.1	26 June
81,041	31.46	23.39	8.07	11.1	8 July
81,049	34.80	29.52	5.28	11.1	10 July
81,085	37.92	26.26	11.66	11.1	23 July
81,087	33.85	29.17	4.68	11.1	24 July
81,109	35.73	27.80	7.93	11.1	2 August
81,110	UD	32.94		ND	3 August
81,135	UD	27.76		11.1	17 August
81,157	UD	33.54		ND	23 August
77,757	34.58	27.68	6.90	12.1	17 June
78,049	UD	34.16		ND	1 August
78,123	37.81	23.56	14.25	11	18 August

^aAssay recommended by the WHO for the detection of all influenza A viruses.

^bModified assay established in this study.

^cValues = (C_T of Aw1) − (C_T of At).

^dIn 2012.

^eUD, M gene of the influenza A virus was undetectable.

^fND, unavailable.